Cleaning of Lab Glassware
Introduction
Proper cleaning of laboratory glassware is a fundamental skill that every lab technician must master. Ensuring glassware is thoroughly clean is crucial for reliable and accurate results in chemical processes, especially in organic synthesis. Clean glassware prevents contamination and guarantees consistent experimental outcomes.

To maintain cleanliness, glassware should be washed immediately after use. Delays can cause residues to harden, making them more difficult to remove. Specialized laboratory detergents like Liquinox or Alconox are preferred over common household dish soaps. In most cases, using detergent and tap water is not ideal; instead, a rinse with an appropriate solvent, followed by distilled and then deionized water, is the recommended protocol.
Effective Cleaning of Lab Glassware Methods
1. Sulfochromic Cleaning Solution for Cleaning of Lab Glassware
A traditional and highly effective method involves using a sulfochromic acid mixture, composed of concentrated sulfuric acid and potassium dichromate. When combined, these chemicals form chromic anhydride (CrO₃), a potent oxidizing agent capable of removing persistent organic residues from lab glassware. It’s also used in other applications like photographic bleaching.

Preparation:
- 15 g potassium or sodium dichromate (K₂Cr₂O₇ or Na₂Cr₂O₇)
- 500 mL concentrated sulfuric acid (H₂SO₄)
Slowly add dichromate to the acid while stirring. Perform this step in a cold water bath using a ceramic or glass container, as the reaction releases heat. Some residue at the bottom is normal.
Usage:
Rinse the glassware with water, fill it with the prepared sulfochromic mixture, and allow it to sit from several minutes to a few days depending on contamination level. Then rinse thoroughly with running water. The solution can be reused until it turns green, indicating conversion of Cr(VI) to Cr(III).
Safety Notes:
Both sulfuric acid and dichromate compounds are highly corrosive and toxic. Use gloves, eye protection, and always work in a fume hood. Never use mouth suction when handling solutions with pipettes—use rubber bulbs instead.
2. Potassium Permanganate Solution
Another strong oxidizing solution involves potassium permanganate (KMnO₄), ideal for removing stubborn organic matter.

Preparation and Use:
- Create a 4% KMnO₄ solution.
- Clean glassware with hot water and a brush.
- Pour the solution into the glassware, then carefully add a small amount of concentrated sulfuric acid (3–5 mL per 100 mL solution). This generates heat and rapidly oxidizes contaminants.
If a brown film of manganese dioxide appears, rinse with a 5% solution of:
- Sodium hydrogen sulfate (NaHSO₄)
- Iron(II) sulfate (FeSO₄) / Mohr’s salt / Oxalic acid (preferred)
Reusability:
Acidified KMnO₄ is typically not reused. Unacidified solutions may be used multiple times.
Safety Precautions:
Follow the same safety protocols as for chromic mixtures. Always wear protective equipment and handle with care.
3. Alkali-Alcohol Cleaning Solution
This is a cost-effective and efficient method to clean glassware contaminated with organic residues.
You’ll Need:
- 60 g sodium or potassium hydroxide (NaOH/KOH)
- 500 mL ethanol
- 60 mL deionized water
Dissolve the hydroxide in water first, then add ethanol. Label the container as “Ethanol/NaOH 5:1 Cleaning Solution.” The solution will become hot, so mix carefully using a glass stirrer.
Application:
Soak glassware for 30 minutes to several hours. Rinse thoroughly with deionized water and dry. Store unused solution in a properly labeled container or dispose of it in chemical waste.
Safety:
Wear lab coat, goggles, and chemical-resistant gloves.
4. Solvent and Detergent Rinsing
Step-by-step:
- Rinse residual organic compounds with 2–3 mL of an appropriate solvent (commonly acetone).
- Acetone effectively removes many organics, but not ionic salts—these are best rinsed with water.
- Follow up with soap and water. Use Alconox or diluted lab detergent to minimize foaming.
- Rinse with distilled water to finish.
If deeper cleaning is required, revert to sulfochromic, permanganate, or alkali-alcohol methods as necessary.

Automatic Dishwasher Use in Cleaning of Lab Glassware
Laboratory dishwashers can be convenient, especially in high-throughput labs. However, they are prone to wear when exposed to aggressive chemicals:
- Rinse glassware before loading.
- Avoid placing heavily soiled or chemically active glassware directly inside.
- Be aware that plastic parts and filters may degrade over time.
- Metal components may corrode in acidic environments.
Use cautiously and consider it a trade-off between time-saving and equipment longevity.

Drying Laboratory Glassware
Quick Drying
Let glassware drip-dry overnight after a distilled water rinse. If needed immediately, rinse with acetone and allow it to evaporate, or use a warm oven or suction from a water aspirator.
Important: Never use compressed air lines to dry glassware—this may contaminate it with oil or moisture.
Oven and Flame Drying
To remove trace water for moisture-sensitive reactions:
- Oven-dry at 110 °C overnight.
- Alternatively, flame-dry using a heat gun or Bunsen burner. Be cautious—glassware becomes extremely hot.

a) Removal of vinyl sleeves, b) Clamped, c) First moments with a flame, d) After the flame.
Safety: Always cool dried glassware in a moisture-free environment (desiccator or inert gas stream) before use.

Drying Tubes
Drying tubes offer a moderate method for maintaining dry conditions. Fill with:
- Anhydrous calcium chloride (CaCl₂) or calcium sulfate (CaSO₄)
Used for basic moisture control in setups not requiring extreme dryness. They can also neutralize acidic gases when filled with basic solids like sodium carbonate (Na₂CO₃).

Cleaning of Lab Glassware Final Tips and Summary
- Clean glassware as soon as possible after use to prevent residue buildup.
- Soak in water if immediate cleaning isn’t feasible.
- Pre-treat new glassware with 1% HCl or HNO₃ if it’s slightly alkaline.
- Avoid extremely hazardous solutions (like Piranha) unless you are experienced and equipped for their safe use.
By following these methods and safety protocols, you can ensure your lab glassware remains in optimal condition, minimizing contamination risks and ensuring consistent experimental outcomes.
Sources
- Sigma-Aldrich – Cleaning Laboratory Glassware 👉 Cleaning Laboratory Glassware – Sigma-Aldrich
- University of Nebraska – Laboratory Glassware Cleaning and Storage 👉 Laboratory Glassware Cleaning and Storage (PDF)
- WebPath (University of Utah) – Acid Cleaning Solutions for Glassware 👉 Acid Cleaning Solutions for Glassware (PDF)
- NIST – Cleaning Precision Glassware (GMP‑7) 👉 Cleaning Precision Glassware (NIST GMP‑7)
- Wikipedia – Chromic Acid 👉 Chromic Acid – Wikipedia